Chm 7 functions in NPC quality control

Figure EV1. Expression levels of Chm7-GFP and truncations. A Tenfold serial dilutions of the indicated yeast strains grown at 23, 30 or 37°C for 2–4 days. B Plot of the percentage of Chm7-GFP foci that colocalize with the NE or with Mps3-mCherry-labeled SPBs. Error bars are SD from the mean from three independent experiments where > 50 Chm7-GFP foci were counted. C Relative levels of GFP fusions of Chm7 and truncations assessed by Western blot with anti-GFP antibody and ECL detection; anti-actin is a loading control. Left and right panels are from same nitrocellulose membrane at same exposure. Note that constructs in left panel are expressed from the endogenous CHM7 promoter, whereas right panel constructs are expressed behind the GPD promoter. Numbers reflect positions of molecular weight (MW) standards. D Western blots (anti-GFP) detecting Chm7-GFP levels in the indicated strains with anti-actin loading control or Ponceau-stained membrane to assess relative protein loads. Source data are available online for this figure.